The International Society for Extracellular Vesicles launches the first massive open online course on extracellular vesicles

Figures & data

Table I. Summary of lectures included in the course.

Lecture #	Title of lecture	Lecturer	Affiliation	Length of lecture
Week 1: Introduction to the course and the field of EVs
Lecture 1	Introduction to the field of EVs	Jan Lötvall	University of Gothenburg, Sweden	13 min
Lecture 2	Introduction to the course	Cecilia Lässer	University of Gothenburg, Sweden	11 min
Lecture 3	The origin of EVs throughout the phylogenetic tree	Yong Song Gho	Pohang University of Science and Technology, Republic of Korea	11 min
Lecture 4	History of exosomes and EVs	Philip Stahl	Washington University in St. Louis, United States	32 min
Quiz 1	Introduction to EVs			9 questions
Week 2: Biogenesis, cargo and uptake of EVs
Lecture 5	Biogenesis and release of EVs	Suresh Mathivanan	La Trobe University, Australia	18 min
Lecture 6	Mechanisms of EV uptake – Part 1	David Carter	Oxford Brookes University, UK	22 min
Lecture 7	Mechanisms of EV uptake – Part 2	David Carter	Oxford Brookes University, UK	23 min
Lecture 8	The protein content of EVs	Suresh Mathivanan	La Trobe University, Australia	23 min
Lecture 9	The RNA content of EVs	Andrew F. Hill	La Trobe University, Australia	35 min
Lecture 10	The lipid content of EVs	Edit I. Buzas	Semmelweis University, Hungary	18 min
Quiz 2	Biogenesis, cargo and uptake of EVs			15 questions
Week 3: Collection and processing of cell culture media and body fluids prior to isolation of EVs
Lecture 11	Cell culture media	Cecilia Lässer	University of Gothenburg, Sweden	13 min
Lecture 12	Blood plasma and serum	Kenneth W. Witwer	Johns Hopkins University, United States	15 min
Lecture 13	Breast milk	Esther Nolte-‘t Hoen	Utrecht University, The Netherlands	8 min
Lecture 14	Urine	Lesley Cheng	La Trobe University, Australia	25 min
Lecture 15	Cerebrospinal fluid	Julie A. Saugstad	Oregon Health & Science University, United States	15 min
Quiz 3	Collection and processing of cell culture media and body fluids prior to isolation of EVs			18 questions
Week 4: Methods for isolating EVs
Lecture 16	Differential ultracentrifugation – Part 1	Cecilia Lässer	University of Gothenburg, Sweden	15 min
Lecture 17	Differential ultracentrifugation – Part 2	Cecilia Lässer	University of Gothenburg, Sweden	16 min
Lecture 18	Density gradient	Su Chul Jang	University of Gothenburg, Sweden	12 min
Lecture 19	Size exclusion chromatography	Rienk Nieuwland	Academic Medical Center, The Netherlands	14 min
Lecture 20	Kit-based precipitation	An Hendrix	Ghent University, Belgium	12 min
Lecture 21	Summary of isolation methods for EVs	Cecilia Lässer	University of Gothenburg, Sweden	6 min
Quiz 4	Methods for isolating extracellular vesicles			11 questions
Week 5: Techniques for characterisation and quantification of EVs
Lecture 22	Electron microscopy – Part 1	Johanna Höög	University of Gothenburg, Sweden	9 min
Lecture 23	Electron microscopy – Part 2	Johanna Höög	University of Gothenburg, Sweden	14 min
Lecture 24	Electron microscopy – Part 3	Johanna Höög	University of Gothenburg, Sweden	6 min
Lecture 25	Cryo-TEM	Alain Brisson	University of Bordeaux, France	14 min
Lecture 26	Atomic-force microscopy	Shivani Sharma	University of California, United States	19 min
Lecture 27	Flow cytometry	Marca H. Wauben	Utrecht University, The Netherlands	25 min
Lecture 28	Nanoparticle tracking analysis	Chris Gardiner	University College London, UK	17 min
Quiz 5	Techniques for characterisation and quantification of EVs			18 questions

Cryo-TEM, cryo-transmission electron microscopy; EVs, extracellular vesicles.