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Future Science OA Volume 1, 2015 - Issue 3
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Preliminary Communication

Portable Nanoporous Electrical Biosensor for Ultrasensitive Detection of Troponin-T

Nandhinee Radha Shanmugam1 Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USAView further author information
,
Anjan Panneer Selvam1 Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USAView further author information
,
Thomas W Barrett2 Department of Veterans Affairs, Oregon Health & Science University, Portland, OR, USAView further author information
,
Steven C Kazmierczak3 Department of Pathology, Oregon Health & Science University, Portland, OR, USAView further author information
,
Milin Nilesh Rana4 Department of Molecular & Cell Biology, University of Texas at Dallas, Richardson, TX, USAView further author information
&
Shalini Prasad1 Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USACorrespondence[email protected]
View further author information
Article: FSO24 | Published online: 17 Jun 2015

Figures & data

Figure 1.  Components of the developed sensor platform.

(A) Assembled sensor platform. (B) SEM micrograph of nanoporous nylon membrane with average pore diameter of 200 nm. (C) Characteristic representation of immunoassay inside nanowells.

cTnT:Cardiac Troponin-T; DSP:Dithiobis succinimidyl propionate; EDL:Electrical double layer; IHP:Inner Helmholtz plane; OHP:Outer Helmholtz plane.

Figure 1.  Components of the developed sensor platform.(A) Assembled sensor platform. (B) SEM micrograph of nanoporous nylon membrane with average pore diameter of 200 nm. (C) Characteristic representation of immunoassay inside nanowells.cTnT:Cardiac Troponin-T; DSP:Dithiobis succinimidyl propionate; EDL:Electrical double layer; IHP:Inner Helmholtz plane; OHP:Outer Helmholtz plane.

Figure 2.  Calibration dose response analysis for cardiac Troponin-T antigen diluted in 7% bovine serum albumin.

Cardiac Troponin-T concentrations in the range of 0.001 pg/ml to 10,000 pg/ml were analyzed for n = 3 replicates. Error bars indicate two standard deviation for n = 3 replicates.

Figure 2.  Calibration dose response analysis for cardiac Troponin-T antigen diluted in 7% bovine serum albumin.Cardiac Troponin-T concentrations in the range of 0.001 pg/ml to 10,000 pg/ml were analyzed for n = 3 replicates. Error bars indicate two standard deviation for n = 3 replicates.
Figure 3.  Regression plot for linearity experiments.
Figure 3.  Regression plot for linearity experiments.

Figure 4.  Interference experiments with triglyceride concentrations in the range of 0 mg/dl–2000 mg/dl.

Triglyceride aliquots with cTnT concentration of (A) 1000 pg/ml; (B) 4000 pg/ml; (C) 7000 pg/ml and (D) 10,000 pg/ml.

cTnT:Cardiac Troponin-T.

Figure 4.  Interference experiments with triglyceride concentrations in the range of 0 mg/dl–2000 mg/dl.Triglyceride aliquots with cTnT concentration of (A) 1000 pg/ml; (B) 4000 pg/ml; (C) 7000 pg/ml and (D) 10,000 pg/ml.cTnT:Cardiac Troponin-T.

Table 1.  Table representing the comparison of cardiac Troponin-T concentrations between electrochemical and chemiluminescent immunoassay detection method.

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