Figures & data
Table 1. Nucleolus-enriched RNAs1
Figure 1. Fluorescence in situ hybridization. - : Cells incubated in buffer prior to hybridization; + : cells pre-incubated with RNase. A: IGF2 mRNA hybridization probes. B: 28 S rRNA probes. C: Scrambled sequence probes (see Materials and Methods). The upper panels in A-C are phase contrast images, the lower panels are the fluorescence microscopy images. Arrows indicate hybridization to IGF2 mRNA in nucleoli. (See also Figure S2).
Figure 2. RT-PCR analysis. RNA was isolated from nucleolar, nucleoplasmic and cytoplasmic fractions and subjected to RT-PCR with the primers indicated in the box.
Table 2. Summary of predicted miRNA Targets in IGF2 mRNA1
Figure 3. Predicted binding sites of miR-206 in the 3′-UTR of IGF2 mRNA.
Figure 4. Predicted binding sites of miR-351 in the 3′-UTR of IGF2 mRNA.
Figure 5. Predicted binding sites of miR-494 and miR-340 in the 3′-UTR of IGF2 mRNA.
Figure 6. Predicted binding sites of miR-664 in the 5′- and 3'-UTRs of IGF2 mRNA.
Figure 7. Comparison of the predicted rat myoblast miR-206:IGF2 mRNA interaction and that of miR-206 and KLF4 mRNA in human cells.Citation23
