Figures & data
Figure 1 Anabaena siamensis is ingested and digested by Aedes aegypti larvae. A second instar larva of A. aegypti ingests (A) Anabaena siamensis (B), excreting from its anus, (C) leaving behind digested filaments and intact heterocysts (D).
Figure 2 Plasmid pMVO, designed for excision by Int via site-specific recombination between the attR and attL sites. The 23.4 kb pMVO carries the four Bti toxin genes (cry4A, cry11A, p20 [twice] and cyt1A) and antibiotic resistance marker nptII. Two promoters were introduced, PpsbA (of the photosystem II's D1) and PA1 (T7 phage early promoter) at the denoted positions. nptII, neomycin/kanamycin resistance gene; ORF all3924, a PCR amplified sequenceCitation24 encoding a probable penicillin amidase (see in http://bacteria.kazusa.or.jp/cyanobase/index.html).
![Figure 2 Plasmid pMVO, designed for excision by Int via site-specific recombination between the attR and attL sites. The 23.4 kb pMVO carries the four Bti toxin genes (cry4A, cry11A, p20 [twice] and cyt1A) and antibiotic resistance marker nptII. Two promoters were introduced, PpsbA (of the photosystem II's D1) and PA1 (T7 phage early promoter) at the denoted positions. nptII, neomycin/kanamycin resistance gene; ORF all3924, a PCR amplified sequenceCitation24 encoding a probable penicillin amidase (see in http://bacteria.kazusa.or.jp/cyanobase/index.html).](/cms/asset/794bf1ba-c5dd-43fe-9015-7ae01ca1fdac/kbie_a_10913087_f0002.gif)