High grade glioblastoma is associated with aberrant expression of ZFP57, a protein involved in gene imprinting, and of CPT1A and CPT1C that regulate fatty acid metabolism

Figures & data

Table 1. Main clinical and pathological characteristics of patients

	Grade II gliomas	Grade IV gliomas
Number of patients	46	34
Age >50 y	9	14
Age <50 y	37	20
Males	30	14
Females	16	20
Presence of seizure	16	7
Increased intracranial pressure	14	18
Mean tumor diameter (>5 cm)	20	20
Mean tumor diameter (<5 cm)	26	14
Radiotherapy	25	21
Chemotherapy	22	22
Median follow up (mo)	80	15
Recurrence	20	30

Figure 1. mRNA expression level box plot charts. The upper chart shows the expression levels of ZFP57, TRIM28 and PTEN in the grade II (GII) and grade IV (GIV) cohorts. The lower chart shows the levels of CPT1A, CPT1B, and CPT1C in the same group of patients. The gray boxes represent Q3–Q2, whites boxes are Q2–Q1. The ends of the whisker are set at 1.5× Interquartile (IQR) above the third quartile (Q3) and 1.5× IQR below the first quartile (Q1). For each data set the number of outliers are included in the tables next to the charts. *P < 0.05.

Figure 2. Regression curves of gene expression analysis. Each graph shows the correlation between two genes in GII and GIV cohorts of patients. Data on equations are in Table 2.

Table 2. Gene expression correlation analysis

ZFP57 GII vs CPT1A GII	Sample size	46
	r coefficient	0.7
	Mean x (xˉ)	9.13
	Mean y (yˉ)	27.52
	Intercept (a)	22.16
	Slope (b)	0.59
	Regression line equation	y = 22.16 + 0.59x
ZFP57 GIV vs CPT1A GIV	Sample size	34
	r coefficient	0.65
	Mean x (xˉ)	37.64
	Mean y (yˉ)	59.65
	Intercept (a)	40.50
	Slope (b)	0.51
	Regression line equation	y = 40.50 + 0.51x
CPT1A GII vs CPT1C GII	Sample size	46
	r coefficient	−0.16
	Mean x (xˉ)	27.52
	Mean y (yˉ)	30.67
	Intercept (a)	36.18
	Slope (b)	−0.2
	Regression line equation	y = 36.18 − 0.20x
CPT1A GIV vs CPT1C GIV	Sample size	34
	r coefficient	−0.71
	Mean x (xˉ)	59.65
	Mean y (yˉ)	15.24
	Intercept (a)	36.72
	Slope (b)	−0.36
	Regression line equation	y = 36.72 − 0.36x
ZFP57 GII vs CPT1C GII	Sample size	46
	r coefficient	0.05
	Mean x (xˉ)	9.13
	Mean y (yˉ)	30.67
	Intercept (a)	30.1
	Slope (b)	0.01
	Regression line equation	y = 30.10 + 0.01x
ZFP57 GIV vs CPT1C GIV	Sample size	34
	r coefficient	−0.75
	Mean x (xˉ)	37.64
	Mean y (yˉ)	15.24
	Intercept (a)	26.31
	Slope (b)	−0.29
	Regression line equation	y = 26.31 − 0.29x
ZFP57 GII vs PTEN GII	Sample size	46
	r coefficient	0.24
	Mean x (xˉ)	9.13
	Mean y (yˉ)	31.35
	Intercept (a)	27.78
	Slope (b)	0.38
	Regression line equation	y = 27.78 + 0.38x
ZFP57 GIV vs PTEN GIV	Sample size	34
	r coefficient	−0.68
	Mean x (xˉ)	37.64
	Mean y (yˉ)	26.08
	Intercept (a)	44.16
	Slope (b)	−0.48
	Regression line equation	y = 44.15 – 0.48x
CPT1A GII vs PTEN GII	Sample size	46
	r coefficient	0.31
	Mean x (xˉ)	27.52
	Mean y (yˉ)	31.35
	Intercept (a)	14.99
	Slope (b)	0.60
	Regression line equation	y = 14.99 + 0.60x
CPT1A GIV vs PTEN GIV	Sample size	34
	r coefficient	−0.71
	Mean x (xˉ)	59.65
	Mean y (yˉ)	26.08
	Intercept (a)	64.64
	Slope (b)	−0.64
	Regression line equation	y = 64.64 – 0.64x