Figures & data
Figure 1 DR is a highly pleiotropic process that influences a variety of biological processes, including physiology, fertility, behavior and life span. While the nature of most of these pathways remains unknown, a molecular framework for at least some aspects of DR-dependent life span regulation can be constructed. Under DR conditions (red), rpd3 is down and dSir2 is upregulated. dSir2 activity inhibits Dmp53 (amongst other targets), leading to a portion of the DR related life span extension seen. DR life span extension is larger than life span extension of rpd3, dSir2 or Dmp53 manipulation. Thus each of these interventions define only a portion of the DR life span extension. Reviewed from reference Citation14.
Figure 2 Heat map of KEGG gene sets from Sir2 and p53 long-lived flies show similarity with a DR signature gene set. Red are gene sets that are statistically significantly upregulated, blue are gene sets that are statistically significantly downregulated and black are gene sets that are not statistically significantly changed. RNA is from 10-day old whole body of females.Citation11 Sir2 are flies expressing Sir2 in adult neurons and p53 are flies expressing DN-Dmp53 in adult neurons. yw, w1118 DR flies are genetically identical to Sir2 flies, but without RU486 and genetically very similar to the DN-Dmp53 flies. KEGG categories and scores are in Supplemental Table 2.
Figure 3 Heat map of KEGG gene sets from DR of female whole body and female head and thorax appear very different. Red are gene sets that are statistically significantly upregulated, blue are gene sets that are statistically significantly downregulated and black are gene sets that are not statistically significantly changed. RNA is from Canton-S (C-S) DR and high calorie fed 10-day old female whole bodiesCitation11 or only heads and thoraces. KEGG categories and scores are in Supplemental Table 3.
Figure 4 Heat map of KEGG gene sets from DR and resveratrol female head and thorax appear very similar. Red are gene sets that are statistically significantly upregulated, blue are gene sets that are statistically significantly downregulated and black are gene sets that are not statistically significantly changed. RNA is from the same cohort of Canton-S (C-S) 10-day old female heads and thoraces on a DR diet, high calorie food or fed resveratrol. KEGG categories and scores are in Supplemental Table 4.
