Vitamin D-insufficiency

Figures & data

Table 1. Laboratory parameters and methods of analysis

Parameter	Method	Manufacturer	Analysis device	Stability at ambient temperature
Vitamin D	ECLIA*	Diasorin	Liaison	3 d
Calcium	Photometric	Roche Diagnostics	Modular	7 d
Alk. Phosphatase	IFCC, liquid 37°C	Roche Diagnostics	Modular	7 d
GGT	Enzymatic	Roche Diagnostics	Modular	7 d
Creatinine	Jaffé	Roche Diagnostics	Modular	7 d
Phosphate	UV-colortest	Roche Diagnostics	Modular	3 d
Parathyroid hormone	ECLIA*	Roche Diagnostics	Modular	2 d

* Electro Chemo Lumineszens Immuno Assay.

Table 2. Number of subjects per life-decade

Decade	< 20–39	40–49	50–59	60–69	70–79	80-~
No. of subjects (N:1310)	231	220	223	230	227	179
m (N: 597)	103 (45%)	89 (41%)	103 (46%)	115 (50%)	108 (48%)	79 (44%)
w (N: 713)	128 (55%)	131 (59%)	120 (54%)	115 (50%)	119 (52%)	100 (56%)

Figure 1. Repartition of the age decades in the vitamin D clusters.

Table 3. Number of subjects per region (postal code)

Postal code	Subjects (N)		25-OH-VitD (ng/ml)	Age (years)
0	159	N	159	154
		Mean	17.19	59.34
		Min.	6.00	21.00
		Max.	57.30	97.00
		Median	14.40	63.00
		SD	9.08	18.06
1	236	N	236	233
		Mean	16.11	57.84
		Min.	6.00	20.00
		Max.	66.80	99.00
		Median	14.45	59.00
		SD	8.24	17.42
2	114	N	113	109
		Mean	15.27	57.12
		Min.	6.00	20.00
		Max.	65.30	93.00
		Median	13.00	57.00
		SD	9.35	17.83
3	134	N	133	132
		Mean	17.86	58.30
		Min.	6.00	20.00
		Max.	55.40	96.00
		Median	14.60	62.00
		SD	10.99	17.81
4	71	N	71	70
		Mean	15.24	55.77
		Min.	6.00	21.00
		Max.	45.10	93.00
		Median	13.40	55.00
		SD	9.33	17.52
5	188	N	188	184
		Mean	15.81	56.39
		Min.	6.00	20.00
		Max.	48.90	93.00
		Median	14.25	57.00
		SD	8.08	18.43
6	106	N	106	104
		Mean	16.92	54.61
		Min.	6.00	20.00
		Max.	44.20	86.00
		Median	14.50	54.50
		SD	8.68	17.43
7	103	N	103	99
		Mean	16.28	56.81
		Min.	6.00	19.00
		Max.	50.70	86.00
		Median	13.50	58.00
		SD	9.66	18.96
8	107	N	106	104
		Mean	14.70	59.85
		Min.	6.00	20.00
		Max.	37.90	89.00
		Median	13.45	64.00
		SD	6.16	18.18
9	125	N	113	124
		Mean	16.22	59.10
		Min.	6.00	22.00
		Max.	54.60	87.00
		Median	13.60	62.00
		SD	8.33	18.19

Table 4. Clinical chemistry

Reference Unit	VitD ng/ml	Ca (2.11–2.63) mmol/l	AP (< 104) U/l	GGT (< 42) U/l	Crea (< 0.96) mg/dl	PO4 (0.87–1.45) mmol/l	PTH (15–65) pg/ml
N	1328	1328	1328	1328	1328	1328	1285
Mean	16.23	2.39	71.73	42.71	0.92	1.49	44.53
Min.	6.00	1.79	20.00	5.00	0.47	0.61	10.18
Max.	66.80	3.96	1738.00	4806.00	4.26	9.82	201.60
Median	14.10	2.38	67.00	25.00	0.88	1.28	40.10
SD	8.82	0.11	51.11	141.00	0.265	0.855	21.31
V	0.54	0.05	0.71	3.30	0.29	0.58	0.48

Table 5. Subject numbers per level of vitamin D and life-decade

25(OH)D (ng/ml)	No of subjects						No. / Decade
> = 30	N	%	m		W		N: 99
			N	%	N	%	< = 39	26	26%
	102	8	39	39	63	61	40–49	17	17%
							50–59	22	22%
							60–69	16	16%
							70–79	11	11%
							> = 80	7	7%
< 30 > = 20	230	17	97	42	133	58	N: 225
							< = 39	52	23%
							40–49	41	18%
							50–59	40	18%
							60–69	38	17%
							70–79	34	15%
							> = 80	20	9%
< 20 > = 7	857	65	424	32	433	33	N: 841
							< = 39	133	16%
							40–49	141	17%
							50–59	138	16%
							60–69	155	18%
							70–79	155	18%
							> = 80	119	14%
< 7	139	10	47	34	92	66	N: 132
							< = 39	19	14%
							40–49	19	14%
							50–59	21	16%
							60–69	19	14%
							70–79	23	17%
							> = 80	31	24%

Table 6. Laboratory results according to 25(OH)D clusters

25(OH)D (ng/ml)	ITEM (normal) unit	PTH (15–65) pg/ml	AP (< 104) U/l	Creatinine (< 0.96) mg/dl	Phosphate (0.87–1.45) mmol/l	GGT (< 42) U/l
> = 30	N	99	61	102	102	102
	Mean	36.08	61.82	0.90	1.68	28.09
	Min.	15.90	33.00	0.53	0.71	8.00
	Max.	134.90	104.00	1.81	7.65	141.00
	Median	32.00	62.00	0.89	1.29	20.00
	SD	15.75	14.18	0.22	1.22	22.20
< 30 > = 20	N	221	135	230	230	230
	Mean	38.14	63.56	0.91	1.47	32.39
	Min.	12.70	32.00	0.52	0.61	6.00
	Max.	140.20	143.00	2.62	6.95	288.00
	Median	36.46	60.00	0.87	1.31	22.00
	SD	15.30	19.28	0.25	0.75	34.71
< 20 > = 7	N	815	439	857	857	857
	Mean	45.29	67.05	0.93	1.45	41.40
	Min.	10.18	20.00	0.47	0.63	5.00
	Max.	186.40	130.00	4.26	9.82	739.00
	Median	41.20	65.00	0.88	1.27	26.00
	SD	20.28	18.53	0.28	0.79	54.46
< 7	N	135	76	139	139	139
	Mean	56.94	79.11	0.88	1.58	78.58
	Min.	16.70	22.00	0.48	0.67	8.00
	Max.	201.60	148.00	1.91	8.80	4806.00
	Median	47.87	80.00	0.84	1.33	26.00
	SD	31.32	25.45	0.24	1.03	410.84