A novel system for cloning human TCRs

Figures & data

Figure 1. The hTEC10 system. Briefly, the cDNAs coding for human T-cell receptor (TCR) α and β chain are amplified from single T cells and cloned into an expression vector, which is then used to transduce the TCR⁻ T-cell line TG40. The antigen specificity of the TCR can be assessed by staining TCR-expressing TG40 cells with MHC tetramers or by monitoring CD69 expression. Of note, the entire procedure can be performed in less than 10 d. Reproduced with permissions from ref. 1.

Kobayashi E, Mizukoshi E, Kishi H, Ozawa T, Hamana H, Nagai T, Nakagawa H, Jin A, Kaneko S, Muraguchi A. A new cloning and expression system yields and validates TCRs from blood lymphocytes of patients with cancer within 10 days. Nat Med 2013; 19:1542 - 6; http://dx.doi.org/10.1038/nm.3358; PMID: 24121927

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