Figures & data
Figure 1 (A) Co-RNA-FISH using probes against MALAT1 (a, green) and Neat1 (a′, red) reveals distribution of MALAT1 and Neat1 RNA in nuclear speckles and paraspeckles respectively. (B) Depletion of MALAT1 (MALAT1-AS) in HeLa cells results in nuclear fragmentation. Note that the fragmented nuclei contain nuclear pores (green) and nuclear lamina (red). DNA is counterstained with DAPI. The bar represents 5 µm.
Figure 2 Model depicting the biogenesis of MALAT1 and the involvement of MALAT1 in alternative splicing regulation. (A) By associating with splicing factors in nuclear speckles and the nucleoplasm, MALAT1 regulates the nuclear distribution and recruitment of splicing factors to pre-mRNA, thereby modulating alternative splicing. the color boxes indicate exons and the lines indicate introns. (B) the nascent MALAT1 transcript is cleaved at its 3′end to generate a mature long MALAT1 nuclear RNA and a small ∼61 nucleotide tRNA-like mascRNA, which is transported into the cytoplasm.Citation147 ‘?’ indicates lack of clear evidence on whether nuclear speckles contain both the precursor and mature long MALAT1 transcripts or only the mature transcript.
