Association between spoligotype-VNTR types and virulence of Mycobacterium bovis in cattle

Figures & data

Figure 1. Macroscopic median (± SEM) of lesions according to spoligotypes.

Table 1. Spoligotypes showing higher and lower macroscopic scores, and most predominant spoligotyes

Spoligotype	Score (median)	SEM	No. of isolates	Characteristics
SB0273	27	4.46	4	Rio Cuarto south Cordoba province, not observed elsewhere in Latin America. Australia
SB0520	18	8.3	3	South Cordoba not observed elsewhere in Latin America
SB0140	13.7	7.4	102	Eu1 family^7
SB0145	9.9	6.3	36	Argentina (France?)

Table 2. Macroscopical scores of VNTRs profiles among predominant spoligotypes

Spoligotype	Score	SD	VNTR	No. of isolates	Score
SB0140	13.7	7.4	7–5–5–4*	15	11 ± 1.8
			6–5–5–4*	13	8 ± 1.51
			6–5–5–1	6	10 ± 2.0
			6–9–5–4*	3	9 ± 2.0
SB0145	9.9	6.3	7–5–5–4*	10	13.1 ± 7.4
			7–9*–5–4*	10	7.24 ± 1.76
			7–5–5–3	6	7.6 ± 3.8

The variants of an integer were marked by an asterisk (*) as previously suggested.¹⁸

Figure 2. Association between macroscopic score median (± SEM) according to VNTRs types only from the major spoligotype SB0140.

Table 3. Macroscopical scoring for individual lesions

Characteristic	SCORE
	0	1	2	3	4
Red halo	Absent	Present	//	//	//
Capsule	Non visible	Visible	//	//	//
Color	//	White–yellow	Yellow	//	//
Calcification	Absent	Present	//	//	//
Presentation	//	Focal (1 granuloma)	Multifocal (> 2)	Miliary	Disseminated
Size (diameter)	//	<0.5 cm	0.5–1 cm	1–5 cm	>5 cm

Table 4. Primers sequences of VNTRs locus

VNTR locus	Primer sequence (5–3)- (labeling)
ETR-A	AAATCGGTCC CATCACCTTC TTA-(FAM) CGAAGCCTGG GGTGCCCGCG ATTT
ETR-B	GCGAACACCA GGACAGCATC AT (JOE)-GGCATGCCGG TGATCGAGTG G
ETR-C	GTGAGTCGCT GCAGAACCTG CAG-(HEX) GGCGTCTTGA CCTCCACGAG TG
ETR-D	CAGGTCACAA CGAGAGGAAG AGC-(FAM) GCGGATCGGC CAGCGACTCC TC

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