Haptoglobin-hemoglobin receptor independent killing of African trypanosomes by human serum and trypanosome lytic factors

Figures & data

Figure 1. Characterization of purified TLF-1 and TLF-2. (A) Superose 6 size exclusion chromatography of TLF-1 and TLF-2. Absorbance profiles (280 nM) of TLF-1 and TLF-2, superimposed on individually ran marker proteins [1, thyroglobulin (660 kDa); 2, apoferritin (480 kDa); 3, conalbumin (67 kDa); 4, ovalbumin (45 kDa)]. (B) Analysis of individual Superose 6 column fractions of TLF-1 and TLF-2 separated on non-denaturing 12% SDS-PAGE and silver stained (top panel). Hpr, apoL1 and IgM were detected by protein gel blot. NA, not analyzed.

Figure 2. In vitro activity of human serum, TLF-1 and TLF-2. TLF-1 resistant (R) and susceptible (S) clonal cell lines of bloodstream form T. b. brucei Lister 427 expressing VSG221, 800 and 060 were prepared as previously described.^24,25 The percentage surviving cells was determined, using phase contrast microscopy, 14 h following the addition of TLF-1, TLF-2 or complete human serum to exponentially growing cultures at 37°C. (A) TLF-1 susceptibility of T. b. brucei 427-221^S (black), T. b. brucei 427-800^S (blue), T. b. brucei 427-800^R (red), T. b. brucei 427-060^S (yellow) and 427-060^R (green). (B) Normal human serum (NHS) susceptibility of T. b. brucei 427-221^S (black), T. b. brucei 427-800^S (blue), T. b. brucei 427-800^R (red), T. b. brucei 427-060^S (yellow) and 427-060^R (green). (C) TLF-2 susceptibility of T. b. brucei 427-221^S (black), T. b. brucei 427-800^S (blue), T. b. brucei 427-800^R (red), T. b. brucei 427-060^S (yellow) and 427-060^R (green).

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