ABSTRACT
Prussian blue modified with copper was electrodeposited on a platinum microelectrode for the amperometric determination of hydrogen peroxide at −50 mV. The Prussian blue-copper layer was stable for 2 h at pH 7.4 and a storage period of 2 months. The Prussian blue-copper modified microelectrodes were used to develop oxidase based microbiosensors for ethanol and lactate. Lactate and alcohol oxidases were immobilized by cross-linking with glutaraldehyde. No interferences were observed from possible interfering compounds up to a concentration of 0.5 mM. The calibration plot of the lactate microbiosensor was linear between 0.005 and 0.2 mM with a limit of detection of 0.0035 mM lactate. The linear dynamic range of the alcohol microbiosensor was between 0.05 and 2 mM and the limit of detection was 0.02 mM ethanol. The developed microbiosensors were used for the analysis of pickled cucumber juice, yogurt, and wine.
Acknowledgments
Paper revision by Dr. Alina Vasilescu is gratefully acknowledged.