Polluted surface water is a repository of antibiotic-resistant Escherichia coli harbouring Plasmid-Mediated Quinolone Resistance (PMQR) determinants

ABSTRACT

We investigated the carriage of PMQR determinants by E. coli from surface water and their antibiotypes. Water samples were collected and analysed from two surface water: Agbowo (AG) and Arulogun (AR) in South-west Nigeria for E. coli, which were identified using PCR detection of uidA. Susceptibility to antibiotics was done using the disc-diffusion method. Detection of PMQR genes in isolates showing complete resistance or intermediate susceptibility to the fluoroquinolone antibiotics was done using primer-specific PCR. Thirty-six E. coli isolates were obtained [AG (20) and AR (16)]. The isolates showed different levels of resistance to the tested antibiotics, with 100% resistance to amoxicillin-clavulanate. None of the isolates was positive for extended spectrum β-lactamase (ESBL) production, but 25 showed complete resistance or intermediate susceptibility to the fluoroquinolone antibiotics. Two isolates each from AG and AR carried qnrA, one isolate carried qnrS, with one each from AG and AR carrying aac(6′)-lb-cr. Four isolates carried oqxAB, but, qnrB and qepA were not detected in any isolate. This study revealed that polluted surface water is a ‘hub’ of antibiotic-resistant E. coli harbouring PMQR genes.

KEYWORDS:

• Surface water
• fluoroquinolone resistance
• E. coli
• antibiotic resistance
• extended spectrum β-lactamase
• plasmid-mediated quinolone resistance (PMQR)

Acknowledgments

The authors are very grateful for the cooperation of the people living beside the surface waters for granting us access to collect samples.

Disclosure statement

No potential conflict of interest was reported by the author(s).