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The effect of propolis on biochemical parameters and antioxidant status of the blood of broiler chickens

El efecto del propóleo en parámetros bioquímicos y el estado antioxidante de la sangre de los pollos de engorde

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Pages 173-178 | Received 22 Mar 2013, Accepted 13 Dec 2013, Published online: 15 Mar 2016
 

Abstract

The aim of this work was to investigate the effects of propolis inclusion to the diet on some serum biochemical parameters, enzymatic activities, and antioxidant status of chickens. Five hundred broiler chickens were used in this study, and these animals were divided into five groups. The first group without any addition served as a control. Experimental groups received propolis extract in feed mixture in various doses (E1: 150 mg/kg; E2: 450 mg/kg; E3: 600 mg/kg; and E4: 800 mg/kg). After 42 days, blood samples were collected. Serum glucose, triglycerides, cholesterol, total protein, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, gamma glutamyl transferase, glutamate dehydrogenase, superoxide dismutase, glutathione peroxidase activities, content of albumins, bilirubin, iron, and copper were determined. Significant increase in serum glucose in all experimental groups (13.40 ± 0.88 in E1, 14.38 ± 0.79 in E2, 13.60 ± 0.31 in E3, and 13.55 ± 0.32 mmol/l in E4 group) when compared to the control (11.93 ± 1.21 mmol/l) and GPx activity in group E1 (614.11 ± 58.63 U/l) versus 540.62 ± 58.18 in the control, 550.99 ± 55.25 in E3, and 519.14 ± 60.21 in E4 were measured. The result suggests that the effective dose of propolis was 150 mg/kg in the diet for chicken, and further increase in dose had no effect on antioxidant parameters.

El objetivo de este trabajo fue investigar los efectos de la inclusión de los propóleos en la dieta en algunos parámetros bioquímicos séricos, actividades enzimáticas y el estado antioxidante de los pollos. Se utilizaron quinientos pollos de engorde en este estudio, los cuales se dividieron en cinco grupos. El primer grupo sin ningún añadido sirvió como control. Los grupos experimentales recibieron extracto de propóleos en la mezcla de alimentación en varias dosis (E1: 150 mg / kg; E2: 450 mg / kg; E3: 600 mg / kg; y E4: 800 mg / kg). Después de 42 días, se recogieron muestras de sangre. Se determinaron la glucosa sérica, triglicéridos, colesterol y proteínas totales, la actividad de aspartato aminotransferasa, alanina aminotransferasa, fosfatasa alcalina, gamma glutamil transferasa, glutamato deshidrogenasa, superóxido dismutasa, glutatión peroxidasa, y el contenido de albúminas, bilirrubina, hierro y cobre. Se observó un sumento significativo de la glucosa en suero en todos los grupos experimentales (13,40 ± 0,88 en E1, 14,38 ± 0,79 en E2, 13,60 ± 0,31 en E3, y 13,55 ± 0,32 mmol / l en el grupo E4) en comparación con el control (11,93 ± 1,21 mmol / l) y la actividad de GPx en el grupo de E1 (614,11 ± 58,63 U / l) frente a 540,62 ± 58,18 en el control, 550,99 ± 55,25 en E3, y 519,14 ± 60,21 en E4. El resultado sugiere que la dosis efictiva de propóleos fue 150 mg / kg en la dieta para el pollo, y un mayor aumento de la dosis no tuvo ningún efecto sobre los parámetros de antioxidantes.

Additional information

Funding

This work was supported by VEGA [grant number 1/0022/13], [grant number 1/0084/12], [grant number KEGA 030SPU-4/2012].

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