Vitamin B₂ (riboflavin) increases drought tolerance of Agaricus bisporus

ABSTRACT

Drought is a stressor for many soil-inhabiting organisms. Although plants have been extensively investigated for drought-adaptive mechanisms, little information is available for fungi. Antioxidants are especially relevant, since desiccation is accompanied by an excessive intracellular production of reactive oxygen species. Riboflavin (vitamin B₂) is one antioxidant regulating drought tolerance in plants. A similar function may exist in fungi. Here, we examined the respiratory and transcriptional responses of Agaricus bisporus to drought and the impact of riboflavin. Mesocosm experiments with four groups were established: hyphae were treated with or without 50 µM riboflavin under drought or no drought conditions. Drought increased riboflavin content in hyphae about 5 times with, but also without, addition of riboflavin. Without addition of riboflavin, fungal respiration decreased by more than 50% at water potentials of about −20 MPa. With addition of riboflavin, respiration remained about 2–3 times higher. The transcriptional responses to only drought or only riboflavin strongly overlapped and were mainly based on factors regulating transcription and translation. This was even stronger in combined treatments. Riboflavin induced protective mechanisms in drought-stressed hyphae. Most pronounced was the methylglyoxal (cytotoxic by-product of glycolysis) detoxifying of lactoylglutathione lyase. Thus, our data suggest a stress-priming function and a role of riboflavin in drought responses of A. bisporus.

KEYWORDS:

• Differential gene expression
• drought stress
• microarray
• riboflavin
• saprotrophic filamentous fungi

ACKNOWLEDGMENTS

We thank G. Rambold and T. Leistner for access to laboratory facilities as well as help with the riboflavin extraction. We also thank M. Hochholzer, K. Söllner, A. Dallinger, and R. Mertel for help with laboratory work. We further thank W. Borken and E. Matzner for critical feedback and discussions.

FUNDING

This work was supported by a Deutsche Forschungsgemeinschaft grant (23-1/23-1).

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