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Review Article

Enzyme-linked immunosorbent assay and its application to the serological diagnosis of fungal infection

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Pages 1-14 | Received 01 Jul 1982, Accepted 15 Sep 1982, Published online: 09 Jul 2009
 

Abstract

With the introduction of the enzyme-linked immunosorbent assay (ELISA), a sensitive technique for detection and quantitation of fungal antibodies and antigens became available. Advantages of ELISA over existing serodiagnostic procedures in medical mycology include the use of objective endpoints, the potential for rapid testing of body fluids and that the equipment necessary to preform basic assays can be both simple and cheap. This review outlines the bases and practical details of the different enzyme-immunoassay techniques and then examines the current status of ELISA procedures that have been applied to particular fungal infections in man and animals.

Résumé

Avec l'introduction de la réaction enzymo-immunologique ELISA, une technique sensible de détection et de mesure des anticorps a été obtenue. Les avantages d'ELISA sur les autres méthodes de sérodiagnostique en mycologie médicale incluent l'utilisation de titres limites objectifs et la possibilité de tester rapidement les différents liquides biologiques du corps; de plus, l'équipement de base nécessaire est simple et bon marché. Cette revue souligne les principes de base et les détails pratiques des différentes méthodes enzymo-immunologiques et examine les techniques courantes des procédés ELISA qui ont été appliqués à certaines infections mycosiques des hommes et des animaux.

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