A 90-day subchronic toxicity study of beta-calcium pyrophosphate in rat

Abstract

β-calcium pyrophosphate has been used as a bone-graft extender. The present study evaluated the toxicity from the subchronic administration of β-calcium pyrophosphate to male and female Sprague-Dawley rats. Animals were divided into two groups consisting of 10 male and 10 female rats each and fed β-calcium pyrophosphate extract (30 mg/kg body weight/day) and saline, 7 days per week for 90 consecutive days. During the experiment, no deaths were observed in any groups, and there were no remarkable changes in clinical signs, body weight, food and water consumption, hematological and serum biochemical parameters, organ weight, and histopathological findings between the control and treated groups. The results show no adverse toxic effects of β-calcium pyrophosphate extract (30 mg/kg body weight/day) for rats of either sex.

Keywords::

• Beta calcium pyrophosphate
• subchronic toxicity
• rat

Acknowledgments

This work was supported by the Common Core Technology Development Program of the Korean Ministry of Commerce, Industry and Energy (#A00-A01-3302-02-1-3). The authors thank Bioalpha.com for technical assistance and providing the β-calcium pyrophosphate used in this study.

Declaration of interest: All authors disclose here that there are no conflicts of interest that could innapropriately have influenced the outcome of the present study.