Simultaneous depletion of albumin and immunoglobulin G by using twin affinity magnetic nanotraps

ABSTRACT

For biomarker discovery, simultaneous removal of albumin and immunoglobulin G (IgG) become more important for both concentrating other species including potential biomarkers and getting rid of their masking effect. In this study, we have proposed a covalent and photosensitive cross-linking conjugation of the biomolecules on nanostructures for the depletion of target proteins from aqueous solution and serum. The effect of concentration, pH, temperature and ionic strength on the adsorption of twin affinity nanotraps based on Cibacron Blue F3GA and Protein A were investigated. The efficiency of albumin and IgG depletion from human serum was performed using sodium dodecylsulfate–polyacrylamide gel electrophoresis.

KEYWORDS:

• Albumin depletion
• IgG depletion
• dye-ligand affinity chromatography
• protein a affinity chromatography
• covalent conjugation

Declaration of Interest

The authors declare no competing financial interest.

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