Could vitamin D protect against high fat diet induced damage in the arcuate nucleus in the rat: histological, immunohistochemical and ultrastructural study

ABSTRACT

Obesity is a serious health issue. As regard, the central nervous system, obesity induces neuronal damage. Vitamin D has well-known anti-inflammatory and neuroprotective effects. To detect if vitamin D protects against damage in the arcuate nucleus induced by a high fat-high fructose diet. Forty adult rats were used, and four groups were formed. Group I (negative control) kept on a standard chow diet for six weeks, Group II (positive control) received vitamin D orally once every other day for six weeks, Group III (high fat-high fructose treated group) was given high fat-high fructose diets for six weeks and Group IV (high fat-high fructose and vitamin D treated group) were given high fat-high fructose diets concomitantly with vitamin D for six weeks. High fat-high fructose diet markedly caused histological changes in arcuate neurons as nuclei appeared darkly stained and shrunken with condensed chromatin, and the nucleolus became less prominent. The cytoplasm appeared rarefied with loss of most of the organelles. An increase in neuroglial cells was noticed. The synaptic area showed sparse degenerated mitochondria and a disrupted presynaptic membrane. A high-fat diet has a damaging effect on arcuate neurons and vitamin D alleviates these effects.

KEYWORDS:

• Arcuate neurons
• GFAP
• high-fat diet
• Vitamin D

Disclosure statement

No potential conflict of interest was reported by the authors.

Declarations

Ethics Approval and Consent to participate

Ethics approval was obtained from the Ethics Committee of Faculty of Medicine, Assiut University, Egypt. All methods were performed following the relevant guidelines and regulations and in compliance with ARRIVE guidelines for the care and use of experimental animals by the Committee for Supervision of Experiment on Animals (CPCSEA) and the National Institute of Health (NIH) Protocol.

Availability of data and material

Available from the corresponding author upon reasonable request.

Author contribution

Hala Mohamed Hassanin: Conceptualization, Methodology, Formal analysis, Investigation, Resources, Writing – Original Draft

Omnia I. Ismail: Conceptualization, Methodology, Formal analysis, Investigation, Resources, Writing – Original Draft.

Additional information

Funding

The author received no funding for this work.