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Ozone: Science & Engineering
The Journal of the International Ozone Association
Volume 43, 2021 - Issue 4
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Research Article

In Vitro Comparison of Antibacterial Effect of Ozonated Water and Ozonated Gas

Felipe Agostinia Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-5199-1882
ORCID Icon,
Melissa Faccinia Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-9253-5496
ORCID Icon,
Francisco Fitarellia Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0001-5677-3203
ORCID Icon,
Mariana Aparecida Lopes Ortizb Department of Microbiology, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-3873-1963
ORCID Icon,
Samira Salmeronc Department of Periodontics, Ingá University Center Uningá. 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-6677-5066
ORCID Icon,
Ricardo Cesar Gobbi Oliveiraa Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-0725-2337
ORCID Icon,
Fabricio Pinelli Valarellia Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-4285-486X
ORCID Icon,
Renata Cristina Oliveiraa Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilORCID Iconhttps://orcid.org/0000-0002-9629-9283
ORCID Icon &
Karina Maria Salvatore Freitasa Department of Orthodontics, Ingá University Center Uningá, 87035-510 Maringá, BrazilCorrespondence[email protected]
ORCID Iconhttps://orcid.org/0000-0001-9145-6334
ORCID Icon show all
Pages 394-400 | Received 17 Jun 2020, Accepted 14 Aug 2020, Published online: 01 Oct 2020
 
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ABSTRACT

This study aimed to compare the antibacterial effect of ozonated water and ozonated gas at different concentrations and exposure times. Staphylococcus aureus and Enterococcus faecalis were exposed to ozonated water or ozonated gas at concentrations of 20, 40 and 60 μg/mL for 1 and 2 minutes. A positive control with bacteria and a chlorhexidine 2% negative control were used. The number of colony-forming units (CFU/mL) was evaluated. The concentrations of 40 and 60 μg/mL were significantly more effective. For the E. faecalis, ozonated gas was significantly more effective than ozonated water. When compared to controls, all ozone concentrations were effective in reducing bacteria.

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