Abstract
High-performance liquid chromatography interfaced with inductively coupled plasma mass spectrometry, and hydride generation-inductively coupled plasma mass spectrometry were used, respectively, to investigate changes in both the chemical form and the concentration of selenium during its bio-incorporation and bio-accumulation into rye seedlings. A 60-fold increase in the total level of selenium in the seedlings (‘control’ biomass = 0.99 mg kg−1, ‘enriched’ biomass = 55.27 mg kg−1) was accompanied by a change from selenite to several organo-selenium forms, with more than 40% being present as selenomethionine. The seedling biomass was dried, ground and used as an ingredient in the production of a fermented sourdough bread (popular in Poland and many Eastern European countries). The selenium in the resulting bread was also characterized in terms of its speciation, as well as its total selenium content (‘control’ bread = 0.06 mg kg−1, ‘enriched’ bread = 3.56 mg kg−1). The breads were then fed to 24 volunteers as part of a human intervention study designed to establish the efficacy of this mode of selenium supplementation. The human study data subsequently showed the bread was a good source of dietary selenium.
Acknowledgements
The authors acknowledge funding from EC Project IC15-CT98-0903. M. A. B. acknowledges KBN Grant No. 6P06G 057 20 for supporting work visits to the UK. The paper is dedicated to Rodger, brother of J. L., who died unexpectedly during the completion of the manuscript.