Determination of bixin and norbixin in meat using liquid chromatography and photodiode array detection

Abstract

The development of an analytical method that enables routine analysis of annatto dye, specifically bixin and norbixin, in meat tissue is described. Liquid-solid extraction was carried out using acetonitrile. Analysis was by HPLC with photodiode array detection using two fixed wavelengths (458 and 486 nm). The possibilities of ion trap mass spectrometry (MS) were also assessed. Method performance characteristics, according to Commission Decision 2002/657/EC, were determined, with recoveries between 99 and 102% and calibration curves being linear in the 0.5–10 mg kg⁻¹ range. The limit of quantification was 0.5 mg kg⁻¹.

Keywords:

• annatto
• bixin
• norbixin
• meat
• liquid chromatography
• photodiode array

Acknowledgements

The authors are grateful to Mieke Naessens and Els Laverge for assistance with experimental work and skillful operation of the chromatographic devices.