Haplogroup diversity in the Indian population using 23 Y-STRs

Abstract

Background

A Y-STR polymorphism study is a convenient tool in molecular anthropology and forensic DNA analysis.

Aim

Through standard ethical procedures, the proposed study explored the genetic scenario in male lineage in Madhya Pradesh, a central Indian state, by Y-STR genotyping and haplogroup studies.

Subjects and methods

Five hundred and eleven unrelated male blood samples were directly amplified, and fragment separation was done using capillary electrophoresis to generate a Y-STR profile for 23 forensic relevant markers through PowerPlex^® Y 23 multiplex system. The different statistical methods were applied for studying the forensic and genetics parameters. Subsequently, population comparison was performed by AMOVA, PCoA, and MDS plot, and Haplogroups were predicted with Whit Athey's haplogroup predictor tool.

Conclusion

These data represented the potential value of the PowerPlex^® Y-23 multiplex system for the forensic and human genetics application in the population of Madhya Pradesh, India. Simultaneously the Haplogroup analysis revealed information about the multi-geographic origin as well as multi-ethnic genetic affinities of the Madhya Pradesh population.

Keywords:

• Y-STR
• haplogroup
• forensic analysis
• population genetics
• Madhya Pradesh

Acknowledgements

We thank all the volunteers who donate their blood samples. We also thank Promega Corporation for providing the PowerPlex^® Y-23 system for this study and Whit Athey for his help during the prediction of the haplogroup.

Ethical approval

The present work has been conducted according to the Ethical norm of the declaration of Helsinki (World Medical Association) (RICKHAM 1964). Written informed consent was obtained from the individuals during the biological sample collection for the purpose of regular casework at the Forensic Science Laboratory. This study was approved by the Institutional Ethics Committee, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, M.P., India-470003 vide letter no. DHSGV/IEC/2021/2/02 of dated 3 November 2021.

Disclosure statement

No potential conflict of interest was reported by the author(s).