Simultaneous determination of benthiavalicarb-isopropyl and its four metabolites in fruits and vegetables using ultrahigh -performance liquid chromatography/tandem mass spectrometry

ABSTRACT

We established an efficient analytical approach for simultaneous determination of benthiavalicarb-isopropyl and its four metabolites (KIF-230-M1, KIF-230-M3, KIF-230-M4, and KIF-230-M5) in fruits and vegetables. Samples were extracted with acidified acetonitrile, with octadecylsilane and graphitised carbon black as sorbent for clean-up, and UHPLC-MS/MS for determination. The approach was determined at spikes of 5, 50, and 500 μg kg⁻¹. The recoveries of the six matrices analyzed ranged from 74.5% to 115.6% with 0.5–12.6% for intra-day RSDr (n = 5), and 0.4–13.8% for inter-day RSD_R (n = 15). The five analytes in all matrices achieved high linearity (R² > 0.9917). The limit of quantification (LOQ) of benthiavalicarb-isopropyl as well as its four metabolites in all matrices was 5 μg kg⁻¹. This analytical approach allows for monitoring residues of benthiavalicarb-isopropyl and its metabolites in vegetables and fruits.

KEYWORDS:

• Benthiavalicarb-isopropyl
• metabolites
• residue
• UHPLC‐MS/MS

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The work was supported by The National Natural Science Foundation of China (32072466) and Sinograin II (CHN 2152, 17/0019 Sinograin II).