Sharka in plums: Diagnostics and management

Abstract

Sharka or plum pox is the most destructive disease of plums and other stone fruits belonging to genus Prunus, worldwide. The disease is incited by plum pox virus (PPV), a member of the genus Potyvirus and family Potyviridae. Timelydetection of PPV in fruit trees is necessary prior to embracing an appropriate management tool for containment of the disease to keep the crop loss sufficiently below the economic threshold level. PPV can be diagnosed by visual examination, particularly, during the period of active growth. Traditionally, cultural methods have been used to isolate and identify this dreaded pathogen. Alternatively, molecular detection techniques are also available which, can generate accurate results rapidly enough to be useful for disease management decisions. With the herald of new frontiers of science such as functional genomics and metabolomics, the development of more cost effective novel tools with exceptional sensitivity in real time is not a distant dream to be realized.

Keywords:

• diagnostic tools
• biological assay
• immunosorbent assay
• polymerase chain reaction
• nucleic acid hybridization