Recent Progress in Enzymatic Synthesis of Sugar Nucleotides

Abstract

Glycosylation is one of the most important reactions in nature as it results in the formation of glycoconjugates with diverse biological functions. Sugar nucleotides serve as the natural donor molecules for the biosynthesis of such glycoconjugates and other carbohydrates. Furthermore, these donor molecules are also indispensable building blocks for the enzymatic synthesis of carbohydrates in vitro using Leloir-type glycosyltransferases. Given such importance, the biosynthetic pathways of sugar nucleotides have been exploited, enabling the development of both chemical and enzymatic approaches to produce these molecules. A survey of recent progress in enzymatic synthesis of common mammalian sugar nucleotides as well as their derivatives is thus presented. As a popular strategy, conjugation of sugar nucleotide synthesis with glycosyltransfer reactions and in vivo production of sugar nucleotides are also included.

Keywords:

• Sugar nucleotides
• Enzymatic synthesis
• Carbohydrates
• Glycosyltransferase

ACKNOWLEDGEMENTS

L.C. acknowledges the startup support from University of South Carolina and USC Salkehatchie. L.C. thanks Robert Woodward from the College of Wooster for editing this manuscript.