NADPH metabolism: a survey of its theoretical characteristics and manipulation strategies in amino acid biosynthesis

Abstract

Reduced nicotinamide adenine nucleotide phosphate (NADPH), which is one of the key cofactors in the metabolic network, plays an important role in the biochemical reactions, and physiological function of amino acid-producing strains. The manipulation of NADPH availability and form is an efficient and easy method of redirecting the carbon flux to the amino acid biosynthesis in industrial strains. In this review, we survey the metabolic mode of NADPH. Furthermore, we summarize the research developments in the understanding of the relationship between NADPH metabolism and amino acid biosynthesis. Detailed strategies to manipulate NADPH availability are addressed based on this knowledge. Finally, the uses of NADPH manipulation strategies to enhance the metabolic function of amino acid-producing strains are discussed.

Keywords:

• NADPH
• amino acid
• metabolic modes
• manipulation strategies
• biosynthetic pathway

Disclosure statement

The authors report no declarations of interest.

Additional information

Funding

This work was financially supported by the National Natural Science Foundation of China [No. 31601459], the Natural Science Foundation of Jiangsu Province [No. BK20150149], China Postdoctoral Science Foundation Grant [No. 2016M590410], and Fundamental Research Funds for the Central Universities [No. JUSRP115A19].