Molecular advancements on over-expression, stability and catalytic aspects of endo-β-mannanases

Abstract

The hydrolysis of mannans by endo-β-mannanases continues to gather significance as exemplified by its commercial applications in food, feed, and a rekindled interest in biorefineries. The present review provides a comprehensive account of fundamental research and fascinating insights in the field of endo-β-mannanase engineering in order to improve over-expression and to decipher molecular determinants governing activity-stability during harsh conditions, substrate recognition, polysaccharide specificity, endo/exo mode of action and multi-functional activities in the modular polypeptide. In-depth analysis of the available literature has also been made on rational and directed evolution approaches, which have translated native endo-β-mannanases into superior biocatalysts for satisfying industrial requirements.

Keywords:

• Endo-β-mannanase
• over-expression
• functional dissection
• enzyme stability
• substrate hydrolysis
• rational designing
• directed evolution

Acknowledgements

We thank Director, CSIR-CFTRI, Mysuru for constant encouragement and support.

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

GSK thank UGC, Govt. of India for the grant of Junior Research Fellowship (JRF). The authors acknowledge the support of the MLP 0116 project funded by CSIR-CFTRI, Mysuru and BSC0112 funded by CSIR, New Delhi.