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Research Articles

Effect of peroxynitrite on human serum albumin: a multi technique approach

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Pages 2066-2076 | Received 15 May 2016, Accepted 22 Jun 2016, Published online: 28 Jul 2016
 

Abstract

In this study, human serum albumin (HSA), the most abundant protein of blood plasma, was modified with varying concentrations of peroxynitrite. The peroxynitrite-induced changes in HSA was monitored by spectroscopy, SDS-PAGE, 1-anilinonaphthalene-8-sulfonic acid (ANS), thermal denaturation studies, and matrix-assisted laser desorption/inonization-time of flight mass spectrometry (MALDI-TOF MS). Aggregate formation was studied by thioflavin T binding and scanning electron microscopy (SEM). The results indicated formation of 3-nitrotyrosine, 6-nitrotryptophan, dityrosine, and carbonyls in modified samples and showed retarded mobility in SDS–polyacrylamide gel. Reduction in α-helicity and surface protein hydrophobicity confirmed the secondary and tertiary structure alterations in peroxynitrite-modified-HSA. Also, attachment of nitro group and increase in melting temperature was observed in modified sample. Furthermore, significant enhancement in the fluorescence intensity of ThT upon binding with peroxynitrite-modified-HSA and images under scanning electron microscope are suggestive of protein aggregation. It is, therefore, speculated that HSA modified by endogenously formed peroxynitrite might act as a trigger for nitration/aggregation and suggested the role of peroxynitrite-modified-HSA in SLE.

Acknowledgements

Facilities provided by the Department of Biochemistry, J. N. Medical College, AMU, Aligarh are greatly appreciated. Authors are also grateful to the Interdisciplinary Biotechnology Unit, AMU, Aligarh for CD analysis of samples and USIF, AMU, Aligarh for SEM analysis of the samples.

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