Probing the interaction between niobium pentoxide nanoparticles and serum albumin proteins by Spectroscopic approaches

Abstract

Nanoparticles (NPs) can directly or indirectly enter into the body because of their small size; then they tend to alter the conformation and function of proteins upon interaction with them. Thus, it is crucial to understand the impact of NPs in a biological medium. Recently, niobium pentoxide nanoparticles (Nb₂O₅ NPs) are finding increasing applications in the biological system, for example, bone tissue and dental material, matrix for biosensing of proteins, etc. In all such applications, the Nb₂O₅ NP interacts with proteins and other biomolecules. Hence, the study of such interactions is of considerable importance. Here in this work, we present the impact of Nb₂O₅ NP on the structure, stability and activity of blood proteins, bovine serum albumin (BSA) and human serum albumin (HSA) by means of various spectroscopic approaches. Steady-state fluorescence studies indicated that intrinsic fluorescence intensities of both serum albumin proteins got quenched upon their interaction with NP. The nature of the quenching was elucidated by time-resolved fluorescence and absorption measurements. Using circular dichroism (CD) and synchronous fluorescence spectroscopy (SFS), the structural perturbations of the protein molecules after interaction with NP were investigated. Moreover, the role of temperature on protein stability upon complexation with NP was also explored. In addition, the effect of NP on protein functionality was probed by esterase-like activity assays.

Communicated by Ramaswamy H. Sarma

Keywords:

• Nanoparticle
• serum albumin proteins
• fluorescence spectroscopy
• circular dichroism spectroscopy
• esterase activity assay

Acknowledgments

We acknowledge NIT Rourkela, Odisha, India, for providing us with laboratory and instrument facilities to conduct this work. We thank Dr. Sirsendu Sekhar Ray. Assistant Professor Grade-I. Department of Biotechnology and Medical Engineering, NIT Rourkela, Odisha for providing niobium pentoxide nanoparticles. We would also like to acknowledge Dr. Hirak Chakraborty, Department of Chemistry, Sambalpur University, Odisha, India for his help during thethe lifetime measurement.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.