Molecular interaction studies of P3CL on bovine serum albumin through biophysical approach

Abstract

In the fields of pharmacology and life sciences, it is essential to study how prescribed drugs interact with carrier proteins in human serum albumin. The current study has evaluated the binding properties of rhodanine derivative; (z)-2-(4-(5-((3-(3-chlorophenyl)-1-phenyl-1H-pyrazol-4-oxo-2-thioxothiazolidin-3-yl)benzamido)acetic acid (P3CL) on bovine serum albumin (BSA) by biophysical approach. BSA is a homology model of Human serum albumin. Due to the cost-effectiveness of Human Serum Albumin (HSA) we have studied the binding properties of rhodanine derivative (P3CL) on BSA. The BSA–P3CL interactions were investigated by fluorescence spectroscopy and revealed the presence of a static quenching mechanism. P3CL possesses good binding affinity on BSA with binding constant K_P3CL = 5.36330 × 10¹³ M⁻¹ binding free energy. We have calculated the binding free energy, the number of binding sites, and the binding constants. The establishment of hydrogen bonds and the active participation of amino acids in drug binding were confirmed by molecular docking studies. As conventional processes for the investigation of pharmacological drugs, therapeutic combinations, and coordinated drug intake, the offered strategies are simple to comprehend, accurate, and rapid to put into practice. Our findings will support an additional investigation into ligand’s pharmacological activity.

Communicated by Ramaswamy H. Sarma

Keywords:

• Rhodanine derivatives
• P3CL
• bovine serum albumin
• binding studies
• biophysical approach
• molecular interactions

Acknowledgements

The authors acknowledge to DST-FIST (SR/FST/LS-1/2018/187), Department of Science and Technology, New Delhi (DST-SHRI – DST/TDT/SHRI/2022/70) and Karpagam Academy of Higher Education for providing the infrastructure facilities for this research work.

Disclosure statement

The authors declare that there is no conflict of interest.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.