Optimized Production of Tannase from Cashew Testa using Aspergillus niger MTCC 5898

ABSTRACT

The production of enzymes by bioprocessing is a good alternative to add value to agro-industry residues. Production cost of enzymes, the major constraint in bioprocessing, may be brought down by multifaceted approaches which include the use of cheap agro-residues for the microbial-based production of the enzyme, and the use of cost-efficient aerobic cell growth strategies and anaerobic-combined strategies like solid state fermentation (SSF). The current study investigated the production of tannase by Aspergillus niger CEPC 11 (MTCC 5898) on an abundantly available waste byproduct cashew testa under solid state A. niger growth. The optimum values of parameters obtained through response surface methodology (RSM) were cashew testa (23%), K₂HPO₄ (3.40 mM), sodium chloride (0.47 mM) and temperature (32–35°C). Optimization of the amount of cashew testa, content of the medium and incubation temperature resulted in a 3.02-fold increase from 97.32–301.70 U/g DS of tannase.

KEYWORDS:

• Aspergillus niger
• cashew testa
• gallic acid
• HPTLC
• Plackett and Burman design
• response surface methodology

Funding

The authors express their sincere thanks to National Agricultural Innovation – Indian Council of Agricultural Research Project (NAIP-ICAR Project – New Delhi, project code 20041/2009) for the financial grant and CEPC Laboratory and Technical Division and Mahatma Gandhi University for providing the necessary facilities for the successful completion of this research work.

Additional information

Funding

The authors express their sincere thanks to National Agricultural Innovation – Indian Council of Agricultural Research Project (NAIP-ICAR Project – New Delhi, project code 20041/2009) for the financial grant and CEPC Laboratory and Technical Division and Mahatma Gandhi University for providing the necessary facilities for the successful completion of this research work.