A Low-Cost Closed-Tube Method for Detection of Adulteration in Ground Meat

ABSTRACT

The increased public concern about adulteration and mislabelling of meat products has fueled a need for the development of fast, reliable, and cost-effective species detection methods. Hence, we developed a high resolution melt analysis (HRMA) for the discrimination of meat species commonly used in the meat industry and their possible adulterants. A universal primer pair spanning a ⁓238 bp fragment of mitochondrial 16S rRNA gene was designed to perform HRMA. The developed workflow was validated on standard meat samples, ternary meat mixtures of eight different species, and 56 commercial meat samples. Distinct melting profiles were generated for each species, several ternary meat mixtures, and commercial food samples. The assay was sufficiently sensitive to detect 0.003 ng/µl of DNA of every targeted species. In short, the developed HRM analysis is a rapid, cost-effective, and efficient species discrimination system for confirmation of meat origin.

KEYWORDS:

• Meat adulteration
• mislabeling
• HRM
• 16S rRNA
• real-time PCR

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data obtained in the present study are available within the article and supplementary materials.

Supplemental data

Supplemental data for this article can be accessed online at https://doi.org/10.1080/08905436.2022.2163250