The Presence of a Cytochrome P450-like Protein in the Human Intestinal Microflora Eubacterium aerofaciens

Gilbert H. John Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

Shannon Walls Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

Ronald Keith Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

Jodi Goodfox-Jones Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

Kayleen Tucker Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

K. J. Abraham Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, Oklahoma 74078, USA

Abstract

Eighteen human intestinal microbial strains were tested for the presence of cytochrome P450, an enzyme involved in detoxifying and toxifying various xenobiotics. The polymerase chain reaction (PCR) technique was used to amplify conserved regions within the gene. From the human intestinal microflora Eubacterium aerofaciens, a DNA fragment of the expected size could be amplified, for which a percentage homology of 20% was determined. Southern blot analysis, using Pseudomonas putida P450 CAM probes, showed reactions with probes at 45 and 55°C. The carbon monoxide (CO) difference spectrum produced a P450-like profile for E. aerofaciens, similar to P. putida P450 CAM. The presence of cytochrome P450 in human intestinal flora may influence the expression of hepatic cytochrome P450s.

Keywords

Human Intestinal Flora Cytochrome P450 Pcr Co Difference Spectrum

The Presence of a Cytochrome P450-like Protein in the Human Intestinal Microflora Eubacterium aerofaciens

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The Presence of a Cytochrome P450-like Protein in the Human Intestinal Microflora Eubacterium aerofaciens

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