Design, formulation and optimization of topical ethosomes using full factorial design: in-vitro and ex-vivo characterization

Abstract

The present study aimed to develop lomefloxacin-loaded ethosomal vesicles intended to be applied topically for treating skin infections. Ethosomes were prepared using the cold method. The formulation variables were optimized using 2² factorial design and Design Expert^® software for analyzing the data statistically and graphically using response surface plots. Phosphatidylcholine (X1) and ethanol (X2) were chosen as the independent variables, while the dependent variables comprised entrapment efficiency (Y1), vesicles size (Y2) and zeta potential (Y3). The optimized ethosomes were subsequently incorporated into Carbopol^® 940 gel and characterized for rheological behaviour, in-vitro release, ex-vivo skin permeation and deposition. The ex-vivo permeation and skin deposition studies showed better results compared to drug solutions. In a nutshell, the ethosomal vesicles were found to be a promising carrier demonstrating enhanced topical delivery of lomefloxacin.

Keywords:

• Topical application
• lomefloxacin hydrochloride
• ethosomes
• rheological behaviour
• ex-vivo permeation

Disclosure statement

No potential conflict of interest was reported by the author(s).