Fabrication and characterization of novel bacterial cellulose/alginate/gelatin biocomposite film

Abstract

Hydrogels from bacterial, algal, and animal cells—bacterial cellulose (BC), alginate, and gelatin, respectively—were combined to fabricate a biocomposite film (BCAGG) via an eco-friendly casting technique. In addition, glycerol was added as a plasticizer to improve the elasticity and water absorption capacity of the film. In this study, BC pellicles were simply deconstructed into fibrils suspension and then reconstructed into films with a supplement of alginate, gelatin and glycerol. The physical appearance of fabricated films resembled native BC but possessed improved ductility, enhanced flexibility, higher water uptake ability and better biocompatibility. The film was found to resist tearing under suture pullout strength in a hydrated state. In vitro cytotoxicity tests showed that the film was cytocompatible. A cell study using a human keratinocyte culture demonstrated enhanced cell adhesion, spreading, and proliferation on the BCAGG film compared with BC/alginate film. The BCAGG film therefore has significant potential for use in biomedical applications, particularly in dermal treatment, skin tissue regeneration, and wound healing.

Keywords:

• bacterial cellulose
• alginate
• gelatin
• glycerol
• composite
• human keratinocyte

Disclosure statement

The authors declare no conflict of interest.

Additional information

Funding

This research was supported by The 100^th Anniversary Chulalongkorn University Fund for Doctoral Scholarship, The 90^th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) and The Graduate School, Chulalongkorn University.