Antimicrobial assay and controlled drug release studies with novel eugenol imprinted p(HEMA)-bacterial cellulose nanocomposite, designed for biomedical applications

Abstract

In this study, a novel bio-composite material that allow sustained release of plant derived antimicrobial compound was developed for the biomedical applications to prevent the infections caused by microorganisms resistant to commercial antimicrobials agents. With this aim, bacterial cellulose (BC)-p(HEMA) nanocomposite film that imprinted with eugenol (EU) via metal chelated monomer, MAH was prepared. Firstly, characterization studies were utilized by FTIR, SEM and BET analysis. Then antimicrobial assays, drug release studies and in vitro cytotoxicity test were performed. A significant antimicrobial effect against both Gram (+) Staphylococcus aureus and Gram (-) Escherichia coli bacteria and a yeast Candida albicans were observed even in low exposure time periods. When antimicrobial effect of EU compared with commercially used agents, both antifungal and antibacterial activity of EU were found to be higher. Then, sustained drug release studies showed that approximately 55% of EU was released up to 50 h. This result proved the achievement of the molecular imprinting for an immobilization of molecules that desired to release on an area in a long-time interval. Finally, the in vitro cytotoxicity experiment performed with the mouse L929 cell line determined that the synthesized EU-imprinted BC nanocomposite was biocompatible.

Keywords:

• Bacterial cellulose
• eugenol
• molecular imprinting
• drug release
• antimicrobial 

Authors’ contributions

Sinem Diken-Gür: Conceptualization, Methodology, Validation, Investigation, Writing-Original Draft, Project administration Nermin Hande Avcioglu: Conceptualization, Methodology, Validation, Investigation, Project administration, Writing-Reviewing and Editing Monireh Bakhshpour-Yücel: Conceptualization, Methodology, Investigation Adil Denizli: Supervision, Resources

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

Derived data supporting the findings of this study are available from the corresponding author on request.

Additional information

Funding

This study was financed by Hacettepe University Scientific Research Projects Coordination Unit (grant number: FHD-2021-19531).