Could erythropoietin reduce the ovarian damage of cisplatin in female rats?

Abstract

The aim of this study is to investigate whether erythropoietin (EPO) can reduce the ovarian damage of cisplatin or not. Thirty, female, Wistar-Albino rats were used in the study. Control group (N = 10): Intraperitoneal saline infusion, Cisplatin group (N = 10): Intraperitoneal 7 mg/kg cisplatin, Cisplatin + EPO group (N = 10): Intraperitoneal 7 mg/kg cisplatin and subcutaneous 200 IU/kg/day EPO. Serum AMH concentrations were measured by enzyme-linked immunosorbent assay kit of AMH. Follicular counts were evaluated according to mean diameter of the follicles. Ovarian damage; including follicular cell degeneration, vascular congestion, hemorrhage, and inflammation was scored histologically using a graduated scale. Posttreatment AMH levels of cisplatin group were significantly lower than control and cisplatin + EPO groups. In cisplatin group, there was a significant decrement in posttreatment AMH level compared to pretreatment AMH level. The total damage score of cisplatin group was significantly higher than scores of control and cisplatin + EPO groups. The mean primordial follicle counts of control and cisplatin + EPO groups were significantly higher than that of cisplatin group (p = .007 and p = .003). The results of this study revealed that EPO administration to cisplatin chemotherapy could ameliorate the ovarian damage. Erythropoietin administration to chemotherapeutic agents might suggest to protect ovarian failure and infertility.

Chinese abstract

本实验的目的是研究促红细胞生成素（EPO）是否能减少顺铂对卵巢的损伤。研究中使用了30只雌性Wistar-Albino大鼠。对照组（N = 10）：腹腔内注射生理盐水, 顺铂组（N = 10）：以7mg/kg剂量腹腔注射顺铂, 顺铂+ EPO组（N = 10）：以7mg/kg剂量腹腔注射顺铂, 并每日皮下注射200IU的EPO。通过酶联免疫吸附测定试剂盒测量血清AMH浓度。根据计数卵泡的平均直径评估卵泡数。包括卵泡细胞退化, 血管充血, 出血和炎症在内的卵巢损伤, 采用分级评分进行组织学评分。顺铂组治疗后AMH水平显著低于对照组和顺铂+ EPO组。在顺铂组中, 与治疗前AMH水平相比, 治疗后AMH水平显着降低。顺铂组总损害评分显著高于对照组和顺铂组+ EPO组。对照组和顺铂+ EPO组的平均原始卵泡计数显著高于顺铂组（p = 0.007和p =0 .003）。这项研究的结果显示顺铂化疗者加用EPO治疗可以改善卵巢损伤。对化疗患者予以促红细胞生成素治疗可能意味着减少卵巢衰竭和不育风险。

Keywords:

• Cisplatin
• erythropoietin
• ovary
• AMH

Acknowledgements

This research was funded by Kırıkkale University Scientific Research Commission (project number: 2015/06). The authors are grateful to Kırıkkale University, for their valuable support.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.

Additional information

Funding

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article.