The modulating effects of Resveratrol on the expression of MMP-2 and MMP-9 in endometriosis women: a randomized exploratory trial

Abstract

Endometriosis is an inflammatory disease; the hallmark of inflammation is over-activation of matrix metalloproteinases (MMPs). The regulatory effects of Resveratrol on MMPs were formerly depicted in other cell lines. This study aimed at investigating the effects of Resveratrol on expression of MMP-2 and -9 in endometriosis patients. This trial was carried out on endometriosis patients (n = 34) who were randomly divided into treatment (i = 17) and control (n = 17) groups. Alongside the routine protocol, the control and treatment groups took placebo and Resveratrol (400 mg), respectively, for 12–14 weeks. Endometrial tissue and fluid as well as blood sampling from both groups were done before and after the intervention. The level of mRNA and protein of both MMP-2 and -9 reduced in the endometrium of treatment group following intervention. Also, the serum and the endometrial fluid concentration of them lowered within the treatment group. Moreover, the serum and endometrial fluid levels of MMP-2 as well as MMP-9 were also diminished following the surgical removal of endometritic lesions. We showed that Resveratrol can modify the inflammation process in the endometrium of women with endometriosis at least in the level of MMP-2 and -9 expressions. The therapeutic potency of Resveratrol in endometriosis needs more clinical studies.

摘要

子宫内膜异位症是一种炎性疾病；炎症的标志是基质金属蛋白酶（MMPs）的过度活化。白藜芦醇对基质金属蛋白酶的调节作用以前在其他细胞系中被描述过。本研究旨在探讨白藜芦醇对子宫内膜异位症患者MMP-2和-9表达的影响。本试验将子宫内膜异位症患者（n = 34）随机分为治疗组（i = 17）和对照组（n = 17）。除常规方案外, 对照组和治疗组分别服用安慰剂和白藜芦醇（400mg）12-14周。在干预前后采集两组的子宫内膜组织和液体并采集血液。干预后治疗组子宫内膜中MMP-2和-9的mRNA水平和蛋白水平均降低。治疗组血清和子宫内膜液中MMP-2和-9的浓度也降低。此外, 在手术切除子宫内膜异位病灶后, 血清和子宫内膜液中MMP-2和MMP-9水平降低。我们发现白藜芦醇能够改变子宫内膜异位症患者的子宫内膜炎性过程, 至少在MMP-2和-9表达水平方面起作用。白藜芦醇在子宫内膜异位症中的治疗效果需要更多的临床研究。

The Chinese abstracts are translated by Prof. Dr. Xiangyan Ruan and her team: Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Keywords:

• Endometriosis
• MMP-2
• MMP-9
• Resveratrol

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was financially supported by Research Deputy of Tehran University of Medical Sciences and Health Services (Grant no. 940230-29327).