Comparison of the protective effects of melatonin and amifostine on radiation-induced epiphyseal injury

Abstract

Purpose: We compared the effects of amifostine and melatonin in preventing radiation-induced epiphyseal growth plate injury in rats.

Materials and methods: Four-week-old (65–85 g), growing male Sprague-Dawley rats were randomly assigned to receive radiation alone, at 25 Gy in three fractions (group R), or this dose of fractionated radiation proceeded by prophylactic amifostine 200 mg/kg i.p. (group A), melatonin 15 mg/kg i.p. (group M), or amifostine + melatonin (group AM). The right rear extremity of each animal was irradiated while the contralateral leg was shielded from radiation, as a control. Bone growth based on the length of the tibia, femur, and overall limb was calculated 6 weeks after the treatment.

Results: In groups R, A, M, and AM, the mean growth loss (GL) for the overall limb was 56.9 ± 8.1%, 46.8 ± 7.7%, 36.6 ± 4.3%, and 38.5 ± 5.1%, respectively. The limb length discrepancies (LLD) in groups R, A, M, and AM were 13.8 ± 1.4%, 10.5 ± 0.3%, 7.4 ± 0.7%, and 8.8 ± 1.1%, respectively. Differences in LLD were significant between each treatment group and group R (range: p = 0.0001–0.001). Differences in either of mean GL and LLD were not significant between groups M and AM; however both of these groups had significantly less GL and LLD than group A.

Conclusions: We observed a superior radioprotective function of melatonin over amifostine in preventing radiation-induced epiphyseal growth plate injury, without any increase in radioprotective effect by adding amifostine to melatonin.

Keywords:

• Amifostine
• melatonin
• fractionated irradiation
• animal
• epiphyseal injury