Advanced search
Publication Cover
International Journal of Radiation Biology Volume 85, 2009 - Issue 9: Special Issue: Papers presented at the Workshop on New Developments in Molecular Imaging in Oncology for Translational Research for Clinical Applications. Guest Editors: Nasreddin Abolmaali, Michael baumann, Robert Bristow and Nicola Sibson
Submit an article Journal homepage
699
Views
44
CrossRef citations to date
0
Altmetric
Original Article

Microscopic imaging of DNA repair foci in irradiated normal tissues

Nirmal Bhogal Applied Molecular Oncology and Radiation Medicine Program, Ontario Cancer Institute/Princess Margaret Hospital (UHN)
,
Farid Jalali Applied Molecular Oncology and Radiation Medicine Program, Ontario Cancer Institute/Princess Margaret Hospital (UHN)
&
Robert G. Bristow Applied Molecular Oncology and Radiation Medicine Program, Ontario Cancer Institute/Princess Margaret Hospital (UHN); Departments of Medical Biophysics and Radiation Oncology, University of Toronto, Ontario, CanadaCorrespondence[email protected]
, MD, PhD, FRCPC
Pages 732-746 | Received 01 Oct 2008, Accepted 27 Jan 2009, Published online: 22 Sep 2009
 
Sample our Medicine, Dentistry, Nursing & Allied Health journals, sign in here to start your FREE access for 14 days

Abstract

Purpose: It is now feasible to detect DNA double strand breaks (DSB) in tissues by measuring the induction and resolution of DNA repair foci, such as γ-H2AX, using immunofluorescent microscopy and digital image analysis. This review will highlight principal tools and approaches to tissue microscopy and analysis. It will also discuss the practical considerations of using microscopy in vitro and in vivo in measuring intranuclear foci following irradiation.

Conclusions: Computer-based image analysis algorithms allow an objective and quantitative analysis of foci and protein-protein interactions using 3D confocal images. Finally, we review the literature in which DNA repair foci have been investigated as a biodosimeter or a biomarker of DNA repair in normal tissues.

Log in via your institution

Access through your institution

Log in to Taylor & Francis Online

Restore content access

Restore content access for purchases made as guest
Purchase options * Save for later

PDF download + Online access

  • 48 hours access to article PDF & online version
  • Article PDF can be downloaded
  • Article PDF can be printed
USD 65.00 Add to cart

Issue Purchase

  • 30 days online access to complete issue
  • Article PDFs can be downloaded
  • Article PDFs can be printed
USD 1,004.00 Add to cart

* Local tax will be added as applicable

Related Research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.