Biocontrol of post-harvest tomato rot caused by Alternaria arborescens using Torulaspora indica

ABSTRACT

An important concern in modern agriculture is the production of healthy foods. This has resulted in an increasing number of studies on alternative plant protection strategies such as biological control. Yeasts have received considerable attention as biological control agents against postharvest fungal diseases of fruits and vegetables. Here, we describe the potential of the yeast strain Torulaspora indica as a biological control agent against Alternaria arborescens, causal agent of tomato fruits decay. In vitro and in vivo assays were used to evaluate the interaction of Torulaspora indica (CCA5S55) and Alternaria arborescens (ABS2). This was followed by the evaluation of putative mechanisms controlling fungal development. Our results suggest that the production of volatile compounds and competition for nutrients are putative mechanisms by which T. indica inhibits A. arborescens growth in vitro. The results of in vivo assays indicate a reduction in the number of A. arborescens-induced lesions in tomato fruits in the presence of T. indica.

KEYWORDS:

• Postharvest
• tomato
• volatile organic compounds
• biocontrol
• sustainable agriculture

Data availability statement

Data supporting the results in the paper are available in the Supplementary Material.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethics approval

No ethical approval was required for this work.

Additional information

Funding

This study was financially supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (grant #2013/02421–3 and #2016/02541–7). This study was also supported by the ‘Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brazil – Brazil (CAPES)’ – Financial Code 001 scholarship to AB.