Storing of exoelectrogenic anolyte for efficient microbial fuel cell recovery

ABSTRACT

Starting up a microbial fuel cell (MFC) requires often a long-term culture enrichment period, which is a challenge after process upsets. The purpose of this study was to develop low-cost storage for MFC enrichment culture to enable prompt process recovery after upsets. Anolyte of an operating xylose-fed MFC was stored at different temperatures and for different time periods. Storing the anolyte for 1 week or 1 month at +4°C did not significantly affect power production, but the lag time for power production was increased from 2 days to 3 or 5 days, respectively. One month storing at –20°C increased the lag time to 7 days. The average power density in these MFCs varied between 1.2 and 1.7 W/m³. The share of dead cells (measured by live/dead staining) increased with storing time. After 6-month storage, the power production was insignificant. However, xylose removal remained similar in all cultures (99–100%) while volatile fatty acids production varied. The results indicate that fermentative organisms tolerated the long storage better than the exoelectrogens. As storing at +4°C is less energy intensive compared to freezing, anolyte storage at +4°C for a maximum of 1 month is recommended as start-up seed for MFC after process failure to enable efficient process recovery.

KEYWORDS:

• Exoelectrogenic culture
• mixed culture storage
• freezing
• refrigerating
• process recovery

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the Academy of Finland [New Indigo ERA-Net Energy 2014; Project no. 283013].