An overview on preparation of enzymes for industrial use

Abstract

Enzymes are specialized proteins that act as catalysts, facilitating chemical reactions by either building up or breaking down molecules in all living organisms. They are distinguished by their specificity, a unique characteristic which has been recognized as a valuable tool by various industries. This specificity allows industries to target very particular processes. For instance, in the food industry, enzymes are used in processes like cheese making, brewing, and baking, where their specific actions are required to produce desired flavours, textures, or shelf-life improvements. While industries highly appreciate the specific functionalities of enzymes, their primary focus lies on the commercial form of the enzyme, which demands to function in rigorous industrial settings, not interfere the processes, and be compatible within the chemical environment. Stability is the deciding factor that greatly influences the enzymatic activity, shelf-life, and consequently, the overall viability and commercial success of enzymes. Thus, incorporating enzymes into industrial processes necessitates their preparation or formulation in a form that meets the specific needs of the intended use. The present work focuses on reviewing the fundamental aspects, requirements, and specifications involved in the preparation of enzymes for selected industrial sectors.

Keywords:

• Enzyme preparation
• industrial enzymes
• formulation
• solid enzyme
• liquid enzyme

Acknowledgements

One of the authors, Shakilanishi S duly acknowledges the support by Council of Scientific Industrial Research (CSIR), Govt. of India, for the Research Associateship and contingency grant [File no. 09/844(0069)2019-EMR-I]. The authors thank the management of Vellore Institute of Technology, Vellore for providing the facilities to carry out the work.

Disclosure statement

The authors have no conflict of interest to declare.

Additional information

Funding

One of the authors, Shakilanishi S duly acknowledges the support by Council of Scientific Industrial Research (CSIR), Govt. of India, for the Research Associateship and contingency grant [File no. 09/844(0069)2019-EMR-I]. The authors thank the management of Vellore Institute of Technology, Vellore for providing the facilities to carry out the work.