Development of Lateral Flow Assays for Rapid Detection of Troponin I: A Review

Abstract

Troponin I as a particular and major biomarker of cardiac failure is released to blood demonstrating hurt of myocardial cells. Unfortunately, troponin I detection in the first hours of acute myocardial infarction usually faces with most negligence. Therefore, developments of point of care devices such as lateral flow strips are highly required for timely diagnosis and prognosis. Lateral flow assays are low-cost paper-based detection platforms relying on specific diagnostic agents such as aptamers and antibodies for a rapid, selective, quantitative and semi-quantitative detection of the analyte in a complex mixture. Moreover, lateral flow assay devices are portable, and their simplicity of use eliminates the need for experts or any complicated equipment to operate and interpret the test results. Additionally, by coupling the lateral flow assay technology with nanotechnology, for labeling and signal amplification, many breakthroughs in the field of diagnostics have been achieved. The present study reviews the use of lateral flow assays in early stage, quantitative, and sensitive detection of cardiac troponin I and mainly focuses on the structure of each type of developed lateral flow assays. Finally, this review summarized the improvements, detection time, and limit of detection of each study as well as the advantages and disadvantages.

Graphical Abstract

Keywords:

• Cardiac failures
• lateral flow assay
• troponin I

Acknowledgments

The authors gratefully acknowledge the University of Medical Sciences, Mashhad, Iran for supporting this work. Y.N. Ertas acknowledges funding support from the 2232 International Fellowship for Outstanding Researchers Program of TÜBİTAK (Project No: 118C346).

Disclosure statement

The authors declare there is no Complete of Interest at this study.