A single-tube multiplex method for monitoring mutations in cysteine 481 of Bruton Tyrosine Kinase (BTK) gene in chronic lymphocytic leukemia patients treated with ibrutinib

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Author contributions

R.Maf. conceived the research, designed the multiplex-PCR and interpreted the results; R.Maf. performed the multiplex-PCR analyses; S.F. and C.G.A. performed the Sanger sequencing analyses; S.M. and P.Z. managed the biological samples; M.M. and F.F. managed and analyzed the clinical data; L.S. and S.B. performed the NGS experiments; V.L. performed the statistical analyses of NGS; R.Maf and R.Mar. supervised the work-flow and wrote the manuscript; P.G., T.T., E.T, L.P., M.L. revised and approved the final version of the manuscript.

Disclosure statement

Authors declare that they have no competing interests related to the present manuscript. Other disclosures are provided in ICMJE form. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.

Additional information

Funding

The authors would like to thank Associazione Italiana per la Ricerca sul Cancro [AIRC IG21436 to R.Mar. and Special Program on Metastatic Disease – 5 per mille #21198 to P.G.], Fondazione Cariplo [TRIDEO 16923 R.Maf.], ERA NET TRANSCAN-2 Joint Transnational Call for Proposals: JTC 2016 [project #179 NOVEL to P.G.] and MIUR for PRIN 2015 ZMRFEA_002 (R.Mar.). S.F. was supported by one-year fellowship from Fondazione Umberto Veronesi. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.