DNA polymorphisms and expression profile of immune and antioxidant genes as biomarkers for reproductive disorders tolerance/susceptibility in Baladi goat

Abstract

The objective of this study was to explore single nucleotide polymorphisms (SNPs) and gene expression of immune and antioxidant markers associated with reproductive disorders in Baladi goats. A total of one hundred adults Baladi does were allocated into two equal-sized groups: normal reproductive performance and does have a history of reproductive disorders. DNA sequencing of PRLR (304-bp), LTF (904-bp), TLR2 (420-bp), TLR4 (335-bp), CLA-DRB3.2 (285-bp), SOD3 (735-bp), CAT (1526-bp), GPX4 (782-bp), and GST (690-bp) revealed SNPs associated with reproductive disorders tolerance/susceptibility in investigated does. Nonetheless, DNA sequencing of beta defensin (483-bp), CCL5 (840-bp), and ATOX1 (374-bp) genes elicited a monomorphic pattern. Levels of PRLR, LTF, TLR2, TLR4, CLA-DRB3.2, beta defensin, and CCL5 genes were significantly up-regulated in does affect with reproductive disorders than tolerant ones; while SOD3, CAT, GPX4, GST and ATOX1 genes pattern elicited an opposite trend. The results herein confirmed the potential significance of SNPs in immune and antioxidant genes as genetic markers for reproductive disorders tolerance/susceptibility in Baladi does. The Gene expression profile of investigated genes could be also used as proxy biomarkers for the prediction of the most susceptible risk time for disease occurrence and for building up an effective management protocol.

Keywords:

• Baladi goat
• antioxidants
• immunity
• single nucleotide polymorphisms
• gene expression
• reproductive disorders

Acknowledgements

The authors acknowledge staff members of the Husbandry and Development of Animal Wealth Department, Faculty of Veterinary Medicine, Mansoura University, Egypt.

Author contributions

Ahmed Ateya conceived, designed the experiment, collected blood samples, performed PCR and wrote the manuscript. Basma Marghani analyzed data and contributed to writing the manuscript. Mona Al-Sharif performed DNA sequencing and contributed to writing the manuscript.

Disclosure statement

None of the authors has any conflict of interest to declare.

Data availability statement

The data that support the findings of this study are available from the corresponding author upon reasonable request. The whole experiment was carried out at the cell level, and no animal experiment was involved. Cell experiments were carried out in accordance with the relevant provisions of the laboratory.

The whole experiment was carried out at the cell level, and no animal experiment was involved. Cell experiments were carried out in accordance with the relevant provisions of the laboratory.

Additional information

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.