Abstract
A 60-day feeding trial was conducted to study the hematology, liver, and intestinal histoarchitecture of Labeo rohita fed with a combination of exogenous enzymes, essential amino acids, and essential fatty acids to DORB (De-oiled rice bran) based diets. Three treatments viz., T1 [DORB + phytase and xylanase (0.01% each)], T2 [DORB + phytase (0.01%) + xylanase (0.01%) + L-lysine(1.4%) + L-methionine (0.4%) + EPA and DHA (0.5%)] and T3 [DORB + phytase (0.01%), xylanase and cellulase (0.075%) + L-lysine (1.4%) +L-methionine (0.4%) + EPA and DHA (0.5%)] were used in the present study. Serum total protein, albumin content and A/G ratio varied significantly (p < 0.05) among groups. Globulin content did not vary significantly among groups (p ≥ 0.05). The Hb content, RBC and MCV count varied significantly (p < 0.05) whereas MCH, MCHC content, WBC and lymphocyte count did not vary significantly among groups (p > 0.05). The liver and intestine examination revealed no visible alteration and showed normal histo-architecture. Based on the finding it is concluded that DORB supplemented with exogenous enzymes, essential amino acids and essential fatty acids with phytase (0.01%), xylanase and cellulase (0.075%), L-lysine (1.4%), DL-methionine (0.4%) and EPA and DHA (0.5%) improves the health of L. rohita.
Acknowledgements
The authors are thankful to the Director, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, for providing all the facilities to accomplish this research work. The authors also thank Vaighai Agro Products Limited, Tamil Nadu, for providing DORB and AB Vista, India, for supplying exogenous enzymes free of cost for the successful completion of this research work.
Ethics statement
The experiment was conducted following the procedures of CPCSEA (Committee for the Purpose of Control and Supervision of Experiments on Animals), Ministry of Environment and Forests (Animal Welfare Division), Govt. of India, on care and use of animals in scientific research. This study was approved by the ethical committee of ICAR-Central Institute of Fisheries Education, Mumbai.
Consent to participate
All the authors agree to participate in this experiment.
Consent for publication
All the authors of this article agree to the publication.
Author contributions
All authors contributed to the study conception and design. Amit Ranjan conducted the experiment, collected and analyzed data, and wrote the original draft of the manuscript. Sarvendra Kumar has assisted the first author in sampling and data collection. Narottam Prasad Sahu has designed the experiment, corrected the draft of the manuscript, and supervised the first author. Ashutosh Dharmendra Deo has helped the first author in the interpretation of data and corrected the draft of the manuscript. Kamal Kant Jain assisted in the data analysis and corrected the draft of the manuscript. All authors have read and approved the final manuscript.
Disclosure statement
The authors declare no competing interests.
Data availability statement
The authors declare that data supporting the results of present study are available within the article.