Detection of genome-wide copy number variation in Murrah buffaloes

Abstract

Riverine Buffaloes, especially the Murrah breed because of their adaptability to harsh climatic conditions, is farmed in many countries to convert low-quality feed into valuable dairy products and meat. Here, we investigated the copy number variations (CNVs) in 296 Murrah buffalo using the Axiom^® Buffalo Genotyping Array 90K (Affymetrix, Santa Clara, CA, USA). The CNVs were detected on the autosomes, using the Copy Number Analysis Module (CNAM) using the univariate analysis. 7937 CNVs were detected in 279 Buffaloes, the average length of the CNVs was 119,048.87 bp that ranged between 7800 and 4,561,030 bp. These CNVs were accounting for 10.33% of the buffalo genome, which was comparable to cattle, sheep, and goat CNV analyses. Further, CNVs were merged and 1541 CNVRs were detected using the Bedtools-mergeBed command. 485 genes were annotated within 196 CNVRs that were identified in at least 10 animals of Murrah population. Out of these, 40 CNVRs contained 59 different genes that were associated with 69 different traits. Overall, the study identified a significant number of CNVs and CNVRs in the Murrah breed of buffalo, with a wide range of lengths and frequencies across the autosomes. The identified CNVRs contained genes associated with important traits related to production and reproduction, making them potentially important targets for future breeding and genetic improvement efforts.

Keywords:

• CNVs
• CNVRs
• QTL
• river buffalo
• traits

Acknowledgments

The data used in this work were provided by the International Buffalo Consortium, see Colli et al 2018. Front. Genet. 9:53. doi: 10.3389/fgene.2018.00053. All the authors of the manuscript wish to thank the International Buffalo Consortium for providing array data of 296 Brazilian Murrah Buffaloes. Support received from the National Agricultural Science Fund (NASF), Indian Council of Agricultural Research, New Delhi, India is duly acknowledged. The authors wish to thank the Director and Joint Director (Research), ICAR-Indian Veterinary Research Institute, Bareilly, India for providing the necessary facilities during this study.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The data used in this work were provided by the International Buffalo Consortium.

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.