Assessment of Radical Scavenging Activity and Estimation of EC50 Values of Various Extracts of Leaves and Roots from Lobelia nicotianifolia Roth. (Wild Tobacco)

ABSTRACT

The antioxidant potential (% RSA and EC₅₀) of solvent extracts of leaves and roots of Lobelia nicotianifolia Roth. was assessed using five in vitro assays. The EC₅₀ values of methanolic extract were comparable to that of ascorbic acid for nitric oxide (30.67 µg mL⁻¹) and hydrogen peroxide (81.01 µg mL⁻¹) radical assays. The % RSA and EC₅₀ correlated with the total phenolic content and total flavonoid content. The characterization by liquid chromatography-high-resolution mass spectrometry showed the presence of five flavonoids, six phenolics, three carotenoids, one anthraquinone, coumarin, hydroxyquinone, and isoflavonoid. Embelin, gallic acid, and quercetin were quantified by high-performance liquid chromatography.

KEYWORDS:

• LC-HRMS
• HPLC

Acknowledgments

The authors are grateful to Mr. Vijay Mohan Crishna (Director, NGCPR) for providing research funds under the in-house funding scheme and Dr. Mayur D. Nandikar (Head and Scientist) for providing the necessary research facilities at NGCPR.

Disclosure Statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was supported by the NGCPR [Lobelia In- house fund (2017-2020)].